Chromatography Resource Center

Part of Thermo Fisher Scientific

Chromatography Webinars

Chromatography Webinars

Learn more about chromatography with our upcoming and archived webinars.

Upcoming Webinars

Our next upcoming webinar will be posted soon.

On-Demand Webinars

How to Improve Your Peptide Quantitation Workflow to Provide Greater Speed and Reproducibility

Peptide mapping and quantitative analysis require workflows which deliver high levels of data quality while balancing the needs of sample throughput and compliance to regulation. During this presentation we will describe how advances at each stage of the workflow, from advanced sample preparation techniques, faster and more efficient separations, combined with highly sensitive HRMS systems and data processing, enable robust, fast, sensitive and highly reproducible workflows.

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Sample Preparation Technologies for Improved Peptide Quantitation Workflow

Peptide quantitative analysis requires workflows that deliver high levels of data quality while balancing the needs of sample throughput and regulatory compliance. During this webinar, Mike Oliver of Thermo Fisher Scientific will describe how advances in sample preparation can provide faster, more reproducible workflows in combination with high data quality and increased confidence in results. He will also touch on specifics within liquid chromatography and Orbitrap-based mass spectrometry.

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The Sweet Revolution in Glycan and Antibody Separations

Monoclonal antibodies and other similar biotherapeutics are playing an ever more important role in the treatment of autoimmune diseases and cancers. It is predicted that within a few years, seven of the top ten pharmaceuticals will be antibodies.

Unfortunately, these biomolecules are extremely hard to characterize, due to an almost infinite number of variants, exacerbated by post translational modifications such as charge variance and aggregation. The number, type and location of glycans adds a further degree of complexity.

We discuss these difficulties and also the current technologies used to maximize glycan separation capabilities and structural elucidation.

Webinar Learning Objectives

  1. Learn about the inherent difficulties in monoclonal antibody characterization
  2. Learn about the latest suite of chromatographic tools available to assist in the characterization
  3. Understand the role of glycosylation in this process, and learn how dedicated chromatography columns can be used to help address the challenge

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Maintaining Chain of Custody from Sample Prep to the Auto Sampler

The ability to be able to successfully defend a laboratory’s data during a court case or audit is paramount to the overall mission of any forensic lab. Central to that is maintaining chain-of-custody throughout the analytical workflow, not just during sample collection, transport, and storage. Laboratories go to great lengths to ensure custody and traceability. One of the last frontiers to maintaining sample custody exists within the analytical workflow, specifically, the labeling of analytical samples. For a large percentage of analyses, the samples are extracted for use in chromatographic instruments, and the samples are stored in 2 mL glass vials.

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New Innovations UHPLC and LCMS workflows for the characterization of mAbs and ADCs

This webinar will discuss new innovations in sample preparation, column technology, UHPLC, and high resolution mass spectroscopy (HRMS) that allow the development of analytical methods with run times of less than 5 minutes for all routine methods.
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Choosing the correct LC Column

This presentation focuses on how to choose the appropriate mode of separation, the correct column and also highlights the importance of the correct mobile phase.
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Don’t Leave it to Chance! Are you Selecting the Correct Consumables for your GC & GC-MS System?
By Ruth Jones (Thermo Fisher Scientific, UK)

Selection of the correct consumables for your GC & GC-MS system is critical in order to ensure that optimum performance is achieved. In many cases, this does not happen, leading to poor chromatography, non-reproducible results and more frequent instrument downtime.
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Next Generation UHPLC Technologies Change the Landscape in LC

This webinar is all about UHPLC performance. How can new column technology, delivering the best separation power, be married with the best UHPLC hardware to ensure that the result is an outstanding one? How can we as chromatographers ensure that even very complex and unfamiliar samples are assayed with the highest scrutiny possible?
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How Robust and Reproducible Micro Elution Sample Preparation Devices Can Help Address the Challenges Within Bioanalyitcal Workflows

Event Overview: Bioanalytical and clinical research laboratories are tasked with providing high quality analytical results from complex biological samples in a high throughput environment while complying with strict legislation. These demands are compounded by the continued drive to higher potancy drugs and long acting formulations which continue to push the required quantification limits to lower levels. The growth of biopharmaceuticals also brings into consideration additional analytical challenges such as solvation and non-specific binding.

In this presentation we will discuss how new developments in the design of micro elution SPE formats can help meet these challenges.

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Optimizing the Performance of Solid Core Column Chromatography for Today and Tomorrow

The use of solid core technology has seen a quiet revolution in the way that chromatography has been performed in many laboratories. However, although this technology has been around for more than 40 years, it is only recently that the design and application of these types of stationary phases have been thoroughly investigated. This has led to a better understanding of how these phases work and also resulted in the design of phases aligned to the structure of the analytes being separated.

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Tips and Tricks – Getting the best out of your GC System

What Does it Cover?
Gas Chromatography is a technique widely used for analysis of matrices such as foods, water and biological samples. In order to get the optimum results from your analysis, it is important to ensure that method development and troubleshooting are addressed concisely.

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Fast, Robust LC and LCMS Workflows for the Comprehensive Characterization of Bio-Therapeutic Proteins

This seminar will discuss new advances in analytical method development with analytical run times below 10 minutes for all routine methods with intelligent, integrated chromatography workflows. Orbitrap technology has been established as the most powerful mass spectrometry technology for protein characterization. Procedures for incorporating this technology into a complete workflow for biopharma analysis will be presented.

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The Use of Solid Core Technology in the Pharmaceutical Environment

It will outline how solid core technology can be readily used to face the challenges within the pharmaceutical sector, looking at both small and large biomolecules.

Choosing an appropriate column will be discussed, with an insight into why solid core works and how to optimize the solid core to porous layer for different forms of molecules. Presented data will also investigate the robustness of this technology when applied to biological extracts found in DMPK departments.
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Principles of GC Column Selection

Whilst the choice of GC columns may seem myriad, the number of chemistries is actually rather limited, and in this webcast you will learn how to select the best stationary phase composition for your application.
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How New Biopharmaceutical Sample Preparation and Bio-LC Column Technology Can Improve Your Results Quality and Workflow Efficiency

In this webinar we will discuss how development’s in sample preparation and bio LC column technology can be utilized to provide improved quality of results combined with increased efficiency and output for the bioanalyitcal laboratory.
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Using New SPE and LC Columns to Improve Your Results Quality and Workflow Efficiency in Regulated Bioanalysis

In this webinar, we will discuss how the combination of next-generation SPE technology and solid core LC columns delivers improved efficiency to regulated bioanalytical workflows. It will be demonstrated that the combination provides higher levels of cleanliness, reproducibility, throughput, sensitivity and quality of results, with lower failure rates.

Using Consumables to Reduce Complexity, Lower Costs and Maximise Method Performance for Gas Chromatography

With the ever increasing array of consumables available for each part of the modern GC instrument it can be difficult to make the right choice for an application. The result of this is that many users stick to whichever consumables they have available and miss the opportunity to make a real improvement to their method performance and robustness.
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Recent Developments in Protein Glycosylation Analysis by HPLC with Charged Aerosol and Mass Spectrometric Detection

It is believed that glycoproteins of biological, diagnostic, or therapeutic interest owe key aspects of their normal function to the oligosaccharides attached to the protein backbone. Variations in the number, type, composition, or linkage pattern of these glycans may influence the efficacy of a bio-therapeutic product. The ability to correctly identify and measure glycans reliably, quickly, and inexpensively is of practical benefit.

This webinar will discuss promising improvements to glycosylation analysis that couple unique HPLC stationary phases with direct measurement of glycans by charged aerosol detection and with flow-splitting for simultaneous mass spectrometry MS-MS detection.

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Sample Preparation Method Optimization for Increased Efficiency in Bioanalytical Workflows

Sample preparation is a fundamental process within the bioanalytical workflow due to the complex nature of biologically based samples. It has considerable consequences for the downstream robustness of the analytical method, quality of data and workflow efficiency.

This presentation will discuss considerations in selecting the correct sample preparation technique for your bioanalytical requirements and how to optimize the protocol to achieve maximum extract cleanliness and reproducibility of the assay to achieve a more efficient workflow.

Topics covered:

  • Comparison of different sample preparation techniques, and identify their applicability to different bioanalytical sample extraction scenarios.
  • Define what compound and matrix considerations need to be taken into account when selecting the most appropriate sample preparation options.
  • How to optimize your sample preparation protocol with different selectivity options that can be utilized to provide maximum extract cleanliness with the benefits in workflow efficiency.

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Achieving Higher Throughput and Productivity in Monoclonal Antibody Characterization

There is an extensive and increasing development pipeline for monoclonal antibody (MAb) therapeutics, which, combined with advances in automation in upstream processes such as cell culture and purification process development is driving a requirement for innovative analytical tools in order to deliver greater productivity.

Thermo Scientific Innovative SureStop 9mm Vials

Be confident that your vials are sealing properly time after time with Thermo Scientific™ National SureStop™9 mm vials. See how a positive feedback stopping point into the design of the vial finish, over tightening of the closure is prevented. SureStop vials are designed as part of the Advanced Vial Closure System (AVCS) and remove any subjectivity around achieving the optimal seal compression when closing a vial. See how SureStop compares to a generic vial and offers optimal sealing and performance.

Improving your LC Analysis by Design

This seminar begins by looking at what key physiochemical properties of the molecules to be separated are important and how this information can be readily obtained and then used to develop a separation.
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Fast GC – Reducing Your Analysis Time, Increasing your Sample Throughput

In this webinar, we will discuss the move from conventional to fast GC through the use of shorter, narrower columns. Consideration in system parameters that need to be made in order to maintain quality of results will be discussed, and a number of applications showing the move to fast GC presented.
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Difficult Life Science Analyses with UHPLC Electrochemical Detection

This webinar focuses on an electrochemical method using the Hypersil BDS column to provide data used for brain research on learning and memory, neurotransmitter and free radical biomarker levels involved in neurodegenerative diseases, and the efficacy of neuroactive therapeutics for the treatment of neurological conditions.
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New Developments in Chromatography for Improved Analytics of Biopharmaceuticals

New workflow-based analytical tools for protein analysis enable these assessments early in the development process. They provide insights that allow better, faster decisions on how to proceed with your development. This webinar will discuss new advances in state-of-the-art chromatography and mass spectrometry solutions using ion-exchange and reversed-phase columns, to support characterization, comparability, similarity, and stability studies required during biotherapeutic and biosimilar drug development and QA/QC.
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The Importance of Selecting the Appropriate Sample Preparation Technique for Maximizing Cleanliness within Bio Analytical and Clinical Research Workflows

There are a number of sample preparation options available to the bio-analyst, offering varying degrees of performance and sample cleanliness. Selection of which, will have a significant impact on workflows and associated productivity. This presentation demonstrates how various techniques affect the complete workflow, this will then allow for an informed decision to be made when selecting the appropriate sample preparation solution.
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An Objective Comparison of Solid Core HPLC Column Performance
In this webinar the performance of a number of solid core HPLC columns from different manufacturers is compared. The experimentally derived parameters used for the comparison are column pressure as a function of flow rate, efficiency, impedance, peak shape as a function of loading and phase characterization. Read More …

Turning Up the Heat: Optimizing Temperature in Liquid Chromatography
This presentation will demonstrate that by using high temperatures effectively these pre-conceptions can be addressed and also introduce the chromatographer into a world of unforeseen benefits. Read More …

Making the Undetectable Detectable - Derivatization in GC Analysis
You will learn about the principles of GC derivatization, why it should be used in analysis and how the technique can be utilised in order to give the chromatography results you desire. Read More …

Stationary Phase and Mobile Phase Selection for Hydrophilic Interaction Liquid Chromatography
In this webinar, a HILIC chromatographic characterization regime that rigorously probes the structure-selectivity relationships between analytes and stationary phases is presented. Read More …

Using Variations in Solid Core Particle Diameter and Pore Size to Improve UHPLC and HPLC Separations
In this webinar, the impact of varying the pore diameter and particle diameter of solid core silica particles on UHPLC and HPLC separations of both small and larger molecules will be described. Read More …

Addressing the needs of Bio-analysis with new SPE technologies - SOLA
This webinar shows how the new SOLA SPE technology provides a significant advancement on conventional sample preparation technologies in providing robust, reproducible results, in combination with lower elution volumes and cleaner extracts. This provides a more efficient, cost effective and reproducible assay time after time. Read More …