Use this online tool to transfer methods from HPLC to UHPLC conditions. The tool will calculate the new gradient table, adjust sample volumes and run times if applicable. Please insert your current method conditions and find out how much time and solvents you could save using UHPLC.

Current Method

Current Column
Peak Details (Critical Pair)
 

Current Method Conditions

UHPLC Method

Planned Column
Peak Details (Critical Pair)
Recommended Method Conditions

* Use this factor to increase the flow rate of the fast LC method. Note: if factor other than 1 is used, the resolution calculation is disabled.

0 bar
0 Hz
0

Current Gradient Table

Step Time (min) %A  %B  %C  %D 
1 100.0
2
3
4
5
6
7
8
9
10
End Time:0.000  
Recommended Reconditioning Time: n.a.
 

Planned Gradient Table

Step Time (min) %A  %B  %C  %D 
1 0.00 100.0
2
3
4
5
6
7
8
9
10
End Time:0.000  
Recommended Reconditioning Time: n.a.

 

 
 

Sample usage savings

Current UsagePlanned UsageSavings
 

Eluent usage and time savings

Current TotalPlanned TotalSavingsThroughput
Eluent Usage
Time
 
 

This calculator tool is optimized for browsers with mobile capability and will not display correctly on legacy browsers such as Internet Explorer 8.0 or older. To effectively use this tool, we recommend using a newer browser such as: Internet Explorer 9.0/10.0/+, Google Chrome, Mozilla Firefox, Safari, or Opera. Due to the size of the calculator tables we recommend a minimum screen size of 480 pixels.