Chromatography Resource Center

Part of Thermo Fisher Scientific

HPLC Method Development Calculator

Many chromatographers are considering moving their current HPLC methods to new columns geometries and particle sizes to improve speed, resolution and/or sensitivity. To help simplify this process we have developed method transfer calculators for isocratic method and gradient method transfer. Just simply enter your current conditions and the new conditions under consideration to see what effects can be achieved.

To learn more about how the calculators work and the formulas used in the calculator, please read the Background Information.